by Guillermo Madero.
Hi Gary,
Please see:
http://docs.moodle.org/27/en/Upgrade_overview
http://docs.moodle.org/27/en/Upgrading
And regarding backups, please do a full site backup:
http://docs.moodle.org/27/en/Site_backup
If I understood correctly, you're worrying about your Moodle 2.4.3 not working after you upgrade MySQL/PHP? I'd say you shouldn't have any issues, but from what version to what version are you upgrading? Please check the requiered MySQL& PHP upgrade pages.
--- Reference
https://php.net/migration55
https://php.net/manual/en/appendices.php
http://dev.mysql.com/doc/refman/5.5/en/upgrading.html
http://dev.mysql.com/doc/refman/5.5/en/upgrading-from-previous-series.html